Toxic Valley 25: Enabling Protection Across NATO

By Capt. Regina KoestersSeptember 30, 2025

110th Chemical Battalion Conducts Decontamination Procedures with Belgian Armed Forces during Toxic Valley 25
U.S. Army Sgt. John Canete and a Belgian Armed Forces Soldier conduct decontamination procedures after sampling and identifying chemical warfare agents under sub terrain, limited visibility conditions during Toxic Valley 25 in Zemianske Kostol’any, Sept. 11, 2025. Toxic Valley 25 is a multinational exercise that enables protection and readiness with NATO Partners and Allies through open-air chemical warfare agent collection and assessment training. (U.S. Army photo by Capt. Regina Koesters) (Photo Credit: Capt. Regina Koesters) VIEW ORIGINAL

ZEMIANSKE KOSTOL’ANY, Slovakia – Deep in a valley in Slovakia, over eight NATO nations donned their protective gear as they prepared to enter the “hot zone.” U.S. Army Soldiers from the 101st Airborne Division (Air Assault), the 110th Chemical Battalion, and the 53rd Civil Support Team from the Indiana Army National Guard had the unique opportunity to work alongside NATO Allies and partners to sample and identify real live chemical warfare agents under strict safety measures.

Chemical, Biological, Radiological, and Nuclear threats continue to rapidly evolve and shape military operations. Exercises like Toxic Valley 25 are critical to enabling protection capabilities, preserving combat power, and ensuring survivability in contested environments.

110th Chemical Battalion Tests Skills under Limited Visibility during Toxic Valley 25
U.S. Army Sgt. John Canete, assigned to the 110th Chemical Battalion, 48th Chemical Brigade, seals a sample of a potential chemical warfare agent under sub terrain, limited visibility conditions during Toxic Valley 25 in Zemianske Kostol’any, Sept. 11, 2025. Toxic Valley 25 is a multinational exercise that enables protection and readiness with NATO Partners and Allies through open-air chemical warfare agent collection and assessment training. (U.S. Army photo by Capt. Regina Koesters) (Photo Credit: Capt. Regina Koesters) VIEW ORIGINAL

Brig. Gen. Chris A. McKinney, Deputy Director of Partnering, Security Cooperation, and Weapons of Mass Destruction United States European Command, spoke on the effects of multinational live agent training at the strategic, operational, and tactical levels during his visit to the CBRN testing and training center used throughout the exercise.

“Toxic Valley brings together so many of our NATO partners in an environment where we’re able to isolate and concentrate on the protection warfighting function to be able to help us achieve some of our objectives,” said McKinney.

101st Airborne Division (Air Assault) Conducts Live Chemical Agent Training with Slovak Armed Forces
U.S. Army Spc. Christopher Jones, assigned to the 2nd Mobile Brigade Combat Team, 101st Airborne Division (Air Assault), conducts decontamination procedures with a Slovak Armed Forces Soldier using an enhanced chemical agent monitor during Toxic Valley 25 in Zemianske Kostol’any, Sept. 9, 2025. Toxic Valley 25 is a multinational exercise that enables protection, readiness, and interoperability with NATO Partners and Allies through open-air chemical warfare agent collection and assessment training. (U.S. Army photo by Capt. Regina Koesters) (Photo Credit: Capt. Regina Koesters) VIEW ORIGINAL

Live chemical agent training allows the U.S. Army to prepare and assess for CBRN threats during the phase of competition below armed conflict. Through this training, CBRN units can better inform commanders and staffs on how to integrate chemical defense into operational planning.

McKinney said, “We participate in this with many of our NATO Allies to be able to perfect the techniques to detect chemical, biological, or radiological agents to allow us to continue to deter and if necessary, defeat, and operate in a CBRN contaminated environment.”

Slovakia serves as the one of the few locations across NATO where multinational forces can routinely train with live chemical warfare agents in an open-air environment. The U.S. Army’s partnership with the Slovak Armed Forces is invaluable as leaders and Soldiers train together to better prepare for potential hazards across all phases of operations.

Indiana Army National Guard Conducts Chemical Warfare Agent Training in Slovakia
U.S. Army Staff Sgt. Carlos DeLuna assigned to the 53rd Civil Support Team, Indiana Army National Guard, dons his M53 mask as he prepares to sample and identify chemical agents during Toxic Valley 25 in Zemianske Kostol’any, Sept. 15, 2025. Toxic Valley 25 is a multinational exercise that enables protection, readiness, and interoperability with NATO Partners and Allies through open-air chemical warfare agent collection and assessment training. (U.S. Army photo by Capt. Regina Koesters) (Photo Credit: Capt. Regina Koesters) VIEW ORIGINAL

Lt. Col. Casey J. Nunn, commander of the 53rd Civil Support Team, emphasized the importance of this partnership and how the exercise significantly increased readiness for his unit.

“Toxic Valley allows us to work together, speaking a common language under a doctrinal framework to execute tactics, techniques, and procedures for CBRN mitigation, which includes characterization, sampling, and identification of potentially hazardous unknown substances,” said Nunn.

Training with live agents simulates real-world exposure scenarios, preparing U.S. and NATO CBRN response teams to be ready to respond to potential crisis and conduct large-scale combat operations in a CBRN environment. Units built confidence and cohesion through execution of each lane, ultimately improving decision-making under pressure.

110th Chemical Battalion Tests Skills under Limited Visibility during Toxic Valley 25
U.S. Army Sgt. Carlos Lemus, assigned to the 110th Chemical Battalion, 48th Chemical Brigade uses the joint joint agent chemical detector to identify potential chemical agents under sub terrain, limited visibility conditions during Toxic Valley 25 in Zemianske Kostol’any, Sept. 11, 2025. Toxic Valley 25 is a multinational exercise that enables protection and readiness with NATO Partners and Allies through open-air chemical warfare agent collection and assessment training. (U.S. Army photo by Capt. Regina Koesters) (Photo Credit: Capt. Regina Koesters) VIEW ORIGINAL

“The CBRN center here in Slovakia allows us to execute 70% of our mission essential tasks in a live agent environment. The stress that puts on our team is great for developing our tactics against real world CBRN threats…and it’s unlike any training that we’ve received elsewhere,” said Nunn.

By training alongside NATO partners and Allies in Slovakia, U.S. forces practiced joint rapid response while standardizing detection and decontamination procedures to sustain operations across nations.